It is allowed to customize the database options beased on your needs. You could select one or multiple database for MS/MS searching. It is noted that if you selected "All Database", the searching may take a quite long time based on your MS/MS spectrum and server loading. Here is a summary on all database option:

Database              Total Records     Unique Compounds    Version/Date
HMDB Experimental     64711             4049                v5 (Sep 4th 2022)
HMDB Predicted        1786690           204969              v5 (Sep 4th 2022)
GNPS                  80802             11852               Aug 30th 2022
MoNA                  1545053           614391              Aug 15th 2022
MassBank              90190             16879               Sep 4th 2022
MINEs                 3009477           537799              Feb 2nd 2023
LipidBlast            1832594           778501              Feb 7th 2023
RIKEN                 12771             1231                Sep 7th 2022
ReSpect               4310              691                 Sep 7th 2022
VaniyaNP              39937             2681                Sep 4th 2022
MSDIAL                1726174           606878              v4.8 (Sep 11th 2022)
BMDMS                 227307            2739                Aug 20th 2022
                            

Users are allowed to use Neutral Loss (NL) database to discover the chemical identity based on MS/MS spectrum. Please fully understand that NL database option is mainly designed to characterize "unknown" compounds. The database is curated based on the algorithm from METLIN-NL. This option should be cautiously selected. If you want to use it, please choose to change the button to "Yes".